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KMID : 1143520170050010058
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Journal of Korean Society of Oral Health Science
2017 Volume.5 No. 1 p.58 ~ p.62
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Distribution and Identification of Gram-Negative Bacteria in Indoor Environment of Houses
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Bae Il-Kwon
Kang Doo-Ho
Kim Seon-Min
Bae Yeong-Min
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Abstract
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Indoor environments of house and family member can become contaminated with potential variety bacteria. The aim of this study is to accesses the identification and distribution of indoor environments of house contaminated bacterial strains. We sampled living room floor, bathroom floor, bathroom knob, bottom of bathtub, refrigerator, refrigerator handle, desk, table, sink, bottom of shoes rack, hand of family member, nose of family member, and toilet bowl. All samples were cultured on blood agar media (BAM) and MacConkey agar media (MAM). The colonies grown on each media were
identified with matrix-assisted laser desorption/ioniazation-time of flight mass spectrometry (MALDI-TOF) using MALDI Biotyper and 16S rDNA sequencing. Fifty-five isolates on BAM and MAM were identified as Acinetobacter soli (n=8), Acinetobacter lwoffii (n=6), Acinetobacter baylyi (n=3), Acinetobacter pittii (n=3), Acinetobacter baumannii (n=3), Acinetobacter junii (n=2), Acinetobacter radioresistnes (n=1), Aeromonas hydrophila (n=3), Aeromonas jandaei (n=1), Enterobacter cloacae (n=5), Enterobacter aerogenes (n=4), Klebsiella pneumoniae (n=1), Moraxella osloensis (n=1), Massilia timonae (n=1), Pseudomonas oryzihabitans (n=5), Pseudomonas aeruginosa (n=2), Pseudomonas mosselii (n=1), Pseudomonas stutzeri (n=1), Raoultella ornithinolytica (n=2), Serratia marcescens (n=2). These results suggest that indoor environments of house and family members were contaminated with variety nomal flora and nosocomial bacteria.
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KEYWORD
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Indoor environments of house, Family members, Bacteria, Contamination
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